Fig. 5
From: miR-450a-2-3p targets ERK(1/2) to ameliorate ISO-induced cardiac fibrosis in mice
Upregulation of miR-450a-2-3p attenuates the cardiac fibrosis caused by ISO. A Heart-to-body weight ratio (n = 6). B Pathological analysis of heart tissues from mice injected with the miR-450-a-2-3p agomir or agomir-NC was performed by HE staining and Masson staining. C The cross-sectional area of cardiomyocytes. D Collagen volume fraction (CVF). E The expression of NPPA and NPPB was detected by RT‒qPCR. F-I, N The expression of miR-450a-2-3p, α-SMA, COL1, COL3 and ERK(1/2) in cardiac tissues of ISO-induced mice was measured using RT‒qPCR. J-M, O-Q Western blot analysis was conducted to examine the expression of key regulatory proteins involved in fibrosis (α-SMA, COL1, COL3 and ERK(1/2)) in ISO-induced mice injected with the miR-450a-2-3p agomir or agomir-NC. *P < 0.05. **P < 0.01. ***P < 0.001. ns means no significance