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Table 1 Forward and reverse primers of 5 genes (1) Housekeeping gene GAPDH, (2,3) SDH subunit C, D. (4) Sirtuin-3 (5) HIF

From: Impairment of electron transport chain and induction of apoptosis by chrysin nanoparticles targeting succinate-ubiquinone oxidoreductase in pancreatic and lung cancer cells

Gene

Forward primer

(/5 ––– /3)

Reverse primer

(/5 ––– /3)

Size

(bp)

GAPDH

GATTCCACCCATGGCAAATTC

CTGGAAGATGGTGATGGGATT

87

SDHC

GATGGAGCGGTTCTGGAATAA

CATGGGAAGAGACCAACTGTAG

85

SDHD

CATTTCTTCAGGACCGACCTATC

AACTTGTCCAAGGCCCAAT

88

Sirtuin3

AGGGACGATGATGTAGCTGA

GGCGATCTGAAGTCTGGAATG

110

HIF

GTCTGCAACATGGAAGGTATTG

GCAGGTCATAGGTGGTTTCT

103