Fig. 3
From: SerpinA3N is a novel hypothalamic gene upregulated by a high-fat diet and leptin in mice
Representative in situ autoradiographs of C57BL/6J mouse brain sections showing serpinA3N expression in a LFD-fed mouse and b HFD-fed mouse. Levels of serpinA3N gene expression in LFD- and HFD-fed mice measured by semi-quantitative in situ hybridisation c in the arcuate nuclei (ARC), d the ventromedial nuclei of the hypothalamus (VMH) and e the dorsomedial nuclei of the hypothalamus (DMH). Two-way ANOVA showed a significant effect of diet P < 0.001 for the ARC, VMH and DMH and time P < 0.001 for the VMH and P < 0.05 for the ARC and DMH with an interaction between diet and time P < 0.05 for the VMH only. One-way ANOVA showed differences between HFD and LFD from 4 weeks onwards for the ARC and for all times tested for the VMH and DMH. *P < 0.05, **P < 0.01, ***P < 0.001 (n = 6)